ab against cxcl8  (Bio-Techne corporation)

Journal: eLife

Article Title: Short-range interactions between fibrocytes and CD8 + T cells in COPD bronchial inflammatory response

doi: 10.7554/eLife.85875

Figure Lengend Snippet: ( A ) Heatmaps showing the expression of differentially expressed genes with p-value <0.05 of chemokines and chemokine receptors in resting tissular tissue-resident memory T-cells (T RM ) and effector memory T-cells (T EM ) from patients with COPD (n=2 independent samples) in comparison with control subjects (n=2 independent samples) (GEO accession GSE61397). Expression values are expressed as Z-score transformed transcript count. ( B ) Migration experiment design. ( C ) Migration of fibrocytes from patients with COPD in response to CD8 + T cells supernatants from control subjects (Ctl NS ) or COPD patients (COPD). n=6 independent experiments ( D ) CCL2, CXCL2, and CXCL8 levels in CD8 + T cells supernatants from non-smoking control subjects (Ctl NS ) or patients with COPD (COPD) using BioPlex (CCL2, CXCL2) or ELISA (CXCL1, 3, 5, 6, 8). n=4 Ctl NS samples, n=4 COPD samples. *p<0.05, Mann-Whitney test. ( E–F ), Migration of fibrocytes from patients with COPD in response to CD8 + T cells supernatants from control subjects (Ctl NS ) or COPD patients (COPD), in the presence of 200 nM Reparixin (+) or corresponding vehicle (−) ( E ), and in the presence of 1 µg/mL blocking antibody for CXCL8 (+) or control antibody (−) ( F ). n=6 independent experiments *p<0.05, Wilcoxon matched pairs test.

Article Snippet: When indicated, supernatants were pretreated for 30 min at 37 °C with blocking Ab against CXCL8 (clone 6217, BioTechne, 1 µg/mL) or respective control Ab.

Techniques: Expressing, Comparison, Transformation Assay, Migration, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Blocking Assay

Journal: eLife

Article Title: Short-range interactions between fibrocytes and CD8 + T cells in COPD bronchial inflammatory response

doi: 10.7554/eLife.85875

Figure Lengend Snippet: Fibrocyte chemotaxis towards CD8 + T cells is mainly due to an increased CXCL8 secretion by CD8 + T cells in COPD lungs, and promotes direct contact between both cell types. This interaction triggers CD8 + T cell proliferation, cytokine production, and cytotoxic activity. The interaction and its consequences might be further increased by a reinforcement of IFN-γ signaling and expression of molecules belonging to the immune synapse, from the fibrocyte side.

Article Snippet: When indicated, supernatants were pretreated for 30 min at 37 °C with blocking Ab against CXCL8 (clone 6217, BioTechne, 1 µg/mL) or respective control Ab.

Techniques: Chemotaxis Assay, Activity Assay, Expressing

Journal: eLife

Article Title: Short-range interactions between fibrocytes and CD8 + T cells in COPD bronchial inflammatory response

doi: 10.7554/eLife.85875

Figure Lengend Snippet:

Article Snippet: When indicated, supernatants were pretreated for 30 min at 37 °C with blocking Ab against CXCL8 (clone 6217, BioTechne, 1 µg/mL) or respective control Ab.

Techniques: Blocking Assay, Recombinant

Journal:

Article Title: Blockade of the EGF Receptor Induces a Deranged Chemokine Expression in Keratinocytes Leading to Enhanced Skin Inflammation

doi:

Figure Lengend Snippet: Immunohistochemistry of CXCL10, CXCL8, and TGF-α in cultures of full-thickness human skin explants. Skin explants were pre-incubated with vehicle alone (n.t.) or 2 μmol/L PD168393, and then stimulated or not with 1000 units/ml IFN-γ. Afterward, skin chops were dried by delicate contact with Whatman paper and snap-frozen. Representative results from three independent experiments. Original magnification, ×100.

Article Snippet: Abs against human CXCL8 and CXCL10 were from R&D Systems.

Techniques: Immunohistochemistry, Incubation